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DNA Methyltransferase 1 (DNMT, MCMT, M.Hsa1, DNA (cytosine-5-)-methyltransferase 1, DNA MTase Mmu1, M.HsaI)

DNA Methyltransferase 1 (DNMT, MCMT, M.Hsa1, DNA (cytosine-5-)-methyltransferase 1, DNA MTase Mmu1, M.HsaI)

Cat no: D3931-63B

Supplier: United States Biological
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Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:32,000. Western Blot: 0.5-1.5ug/ml. A band at ~170kD band observed in lysates of cell line Jurkat. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See D3931-63B-P. Antibody
Catalogue number: D3931-63B
Reactivities: Human, Mouse, Rat, Bovine, Canine, Porcine
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by affinity chromatography.
Alternative names: DNMT1; DNA (cytosine-5-)-methyltransferase 1; CXXC9; DNMT; FLJ16293; MCMT; MGC104992; DNA methyltransferase 1
References: Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline., Dion V, Lin Y, Hubert L Jr, Waterland RA, Wilson JH., Hum Mol Genet. 2008 May 1;17(9):1306-17. Epub 2008 Feb 5.
Additional info: Recognizes human DNMT1. Species Crossreactivity: mouse, rat, canine, bovine, porcine.

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