DNA Methyltransferase 3A (Dnmt 3A, M.HsaIIIA)

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.\n\nPositive Control: HCT116 cell lysates\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3931-70J

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

As reported

P Type

Pab

Purity

Serum

References

1. Beaulieu, N., et al. An Essential Role for DNA Methytransferase DNMT3b in Cancer Cell Survival. J. Biol. Chem. 277(31):28176-28181, 2002. (Western blot, human)\n2. Robert, MF., et al. DNMT1 is Required to Maintain CpG Methylation and Aberrant Gene Silencing in Human Cancer Cells. Nat. Genet. 33:61-65, 2003. (Western blot, human)\n3. Weisenberger, D.J., et al. Identification and characterization of alternately spliced variants of DNA methyltransferase 3a in mammalian cells. Gene. 298:91-99, 2002. (Human/mouse)\n4. Reynolds, P. A., Sigaroudinia, M., Zardo, G., Wilson, M. B., Benton, G. M., Miller, C. J., Hong, C., Fridlyand, J., Costello, J. F., and Tlsty, T. D. (2006) Tumor Suppressor p16INK4A Regulates Polycomb-mediated DNA Hypermethylation in Human Mammary Epithelial Cells, 281, 24790-24802.

Additional Info

Specific for human DNMT3a protein. Other species have not been tested.

Alternative Names

DNMT3A2, M.HsaIIIA