DNA Methyltransferase 3B (Dnmt 3B)

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.\n\nPositive Control: HCT116 cell lysates.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3931-73G

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

As reported

P Type

Pab

Purity

Serum

References

1. Beaulieu, N., et al. An Essential Role for DNA Methytransferase DNMT3b in Cancer Cell Survival. J. Biol. Chem. 277(31): 28176-28181, 2002. [Western Blot, Human]\n2. Robert, MF., et al. DNMT1 is Required to Maintain CpG Methylation and Aberrant Gene Silencing in Human Cancer Cells. Nat. Genet. 33: 61-65, 2003. [Western Blot, Human]\n3. Qui, Y., et al. Differential expression of DNA-methyltransferases in drug resistant murine neuroblastoma cells. Can. Detect. and Prevent. 26: 444-453, 2002. [Western Blot, Immunofluorescence, Mouse]\n4. Rhee, I., et al. DNMT1 and DNMT3b cooperate to silence genes in human cancer cells. Nature. 416: 552-556, 2002. [Western Blot, Human]\n\n5. Weisenberger, D., et al. Identification and characterization of alternatively spliced variants of DNA methyltransferase 3a in mammalian cells. Gene. 298: 91-99, 2002. [Human, Mouse]\n6. Morey, S. R., et al. DNA Methylation Pathway Alterations in an Autochthonous Murine Model of Prostate Cancer. 66: 11659-11667, 2006.

Additional Info

This antibody is specific for DNMT3b. Species Crossreactivity: Reacts with human and mouse protein. Other species have not been tested.

Alternative Names

ICF, M.HsaIIIB