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DNA Methyltransferase 3B (Dnmt 3B)

DNA Methyltransferase 3B (Dnmt 3B)

Cat no: D3931-73J

Supplier: United States Biological
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Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome. Cellular Localization: Nuclear Positive Control: P19 nuclear extracts. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D3931-73J
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ug
Form: PBS with 1 mg/ml BSA containing 0.05% sodium azide
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: ICF, M.HsaIIIB
References: Okano M et al. DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell 99:247-57 (1999).
Additional info: This antibody detects DNA methyltransferase 3b (Dnmt3b) from human and mouse tissues and cells as well as recombinant human Dnmt3b. This antibody detects, to a lesser extent, full-length human recombinant Dnmt3a. Species Crossreactivity: Crossreacts with Human and Mouse.

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