DNA Methyltransferase 3L (Dnmt 3L, DNA Cytosine 5

DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have\ndemonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.\n\nApplications: \nSuitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1000-1:3000\nWestern Blot: 1:1500. Detects human DNMT3L by Western Blot after Immunoprecipitation. Transfection of U2OS cells\n(10cm dish) was accomplished using 3ug of GAL4-DNMT3L. Protein extraction using IPH at 150mM. Does not perform well when applied directly for Western Blot. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile (40-50% ) glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3931-76

Size

100ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Suetake I, Shinozaki F, Miyagawa J, Takeshima H, Tajima S. (2004) DNMT3L stimulates the DNA methylation activity of Dnmt3a and Dnmt3b through a direct interaction. J Biol Chem. Apr 21 [Epub ahead of print] Lucifero D. et al. (2004) Gene-specific timing and epigenetic memory in oocyte imprinting. Hum Mol Genet. 13(8):839-49. 2. Gribnau J, Hochedlinger K, Hata K, Li E, Jaenisch R. (2003) Asynchronous replication timing of imprinted loci is independent of DNA methylation, but consistent with differential subnuclear localization. Genes Dev. 17(6):759-73. 3. Chedin F, Lieber MR, Hsieh CL. (2002) The DNA methyltransferase-like protein DNMT3L stimulates de novo methylation by Dnmt3a. Proc Natl Acad Sci U S A. 99(26):16916-21. 4. Deplus R. et al. (2002) Dnmt3L is a transcriptional repressor that recruits histone deacetylase. Nucleic Acids Res. 30(17):3831-8. Aapola U, Liiv I, Peterson P. (2002) Imprinting regulator DNMT3L is a transcriptional repressor associated with histone deacetylase activity. Nucleic Acids Res. 30(16):3602-8. 5. Bourc'his D, Xu GL, Lin CS, Bollman B, Bestor TH. (2001) Dnmt3L and the establishment of maternal genomic imprints. Science. 294(5551):2536-9. 6. Aapola U, Lyle R, Krohn K, Antonarakis SE, Peterson P. (2001) Isolation and initial characterization of the mouse Dnmt3l gene. Cytogenet Cell Genet. 92(1-2):122-6. 7. Aapola U. et al. (2000) Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family. Genomics. 65(3):293-8. 8. Fuks F, Burgers WA, Brehm A, Hughes-Davies L, Kouzarides T.(2000) DNA methyltransferase Dnmt1 associates with histone deacetylase activity. Nat Genet. 24(1):88-91.

Additional Info

Recognizes Human DNMT3L MW: 43.6kD. Species Sequence Homology: Mouse: 76% .