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DNA PK, Catalytic Subunit (DNA-dependent Protein Kinase)

DNA PK, Catalytic Subunit (DNA-dependent Protein Kinase)

Cat no: D3949-01H

Supplier: United States Biological
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DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damage. Cellular Localization: Nuclear Positive Control: LS174T cells. Tonsil. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D3949-01H
Reactivities: Human, Rat
Hosts: Mouse
Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 500ul
Form: 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Alternative names: DNAPK, DNAPK1, DNA-PKcs, DNA-Dependent Protein Kinase, catalytic subunit, PRKDC, Protein Kinase, DNA-activated, catalytic subunit, HYRC, HYRC1, Hyper-Radiosensitivity Complementing 1, XRCC7, X-ray Repair Complementing Defective Repair in Chinese Hamster Cells 7, p350, p460
References: Han Z et al. DNA-dependent protein kinase is a target for a CPP32-like apoptotic protease. J Biol Chem 271:25035-40 (1996). Kirchgessner CU et al. DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science 267:1178-83 (1995). Poltoratsky VP et al. Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases. J Immunol 155:4529-33 (1995). Konno-Sato S et al. Phosphorylation of a 72-kDa nucleoprotein (NP-72) in HL-60 cells is mediated by the double-stranded DNA-dependent protein kinase (DNA-PK). Biochem Mol Biol Int 31:113-24 (1993). Carter T et al. A DNA-activated protein kinase from HeLa cell nuclei. Mol Cell Biol 10:6460-71 (1990).
Additional info: This antibody is specific to 460kD protein known as DNA-dependent Protein Kinase (DNA-PKcs). Species Crossreactivity: Crossreacts with Human and Rat.

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