DNA PK, Catalytic Subunit (DNA-dependent Protein Kinase)

DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damage.\n\nCellular Localization: Nuclear\n\nPositive Control: LS174T cells. Tonsil.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

D3949-01H

Size

500ul

Applications

IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

Han Z et al. DNA-dependent protein kinase is a target for a CPP32-like apoptotic protease. J Biol Chem 271:25035-40 (1996). <PUBMED:8798786>Kirchgessner CU et al. DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science 267:1178-83 (1995). <PUBMED:7855601>Poltoratsky VP et al. Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases. J Immunol 155:4529-33 (1995). <PUBMED:7594449>Konno-Sato S et al. Phosphorylation of a 72-kDa nucleoprotein (NP-72) in HL-60 cells is mediated by the double-stranded DNA-dependent protein kinase (DNA-PK). Biochem Mol Biol Int 31:113-24 (1993). <PUBMED:8260934>Carter T et al. A DNA-activated protein kinase from HeLa cell nuclei. Mol Cell Biol 10:6460-71 (1990). <PUBMED:2247066>

Additional Info

This antibody is specific to 460kD protein known as DNA-dependent Protein Kinase (DNA-PKcs). Species Crossreactivity: Crossreacts with Human and Rat.

Alternative Names

DNAPK, DNAPK1, DNA-PKcs, DNA-Dependent Protein Kinase, catalytic subunit, PRKDC, Protein Kinase, DNA-activated, catalytic subunit, HYRC, HYRC1, Hyper-Radiosensitivity Complementing 1, XRCC7, X-ray Repair Complementing Defective Repair in Chinese Hamster Cells 7, p350, p460