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Products DNA PK, Catalytic Subunit, phosphorylated (Thr2609) (DNA-dependent Protein Kinase)
| Catalogue number: | D3949-01K |
| Reactivities: | Human |
| Hosts: | Rabbit |
| Applications: | Western Blot |
| Size: | 100ug |
| Form: | As reported |
| P type: | Pab |
| Isotype: | IgG |
| Purity: | Purified by immunoaffinity chromatography. |
| Alternative names: | DNAPK, DNAPK1, DNA-PKcs, DNA-Dependent Protein Kinase, catalytic subunit, PRKDC, Protein Kinase, DNA-activated, catalytic subunit, HYRC, HYRC1, Hyper-Radiosensitivity Complementing 1, XRCC7, X-ray Repair Complementing Defective Repair in Chinese Hamster Cells 7, p350, p460 |
| Additional info: | This phospho specific polyclonal antibody reacts with phosphorylated pT2609 of human DNA PKcs. Reactivity with non-phosphorylated human DNA PKcs is minimal by ELISA.This antibody recognizes phospho T2609 DNA PKcs as demonstrated: IPed DNA PKcs (using another DNA PKcs antibody) blotted with the pT2609 antibody shows an inducible signal at the correct height after DNA damage. However, unfortunately the antibody picks up an IR-inducible signal in cells lacking DNA-PKcs (MO59J cells) at the same size as DNA-PKcs. We believe that this is 53BP1 which runs at the same size as DNA-PKcs and is also phosphorylated in an IR-dependent manner. Similar results were seen with another phospho DNA-PKcs antibody indicating that this result is general to antibodies to this phospho site, rather than specific to this antibody. This antibody may be a useful marker for DNA damage. It may also still be useful in the study of phospho DNA PKcs T2609. Species Crossreactivity: Crossreacts with Human. |
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