DNA-PKcs (DNA PKcs pT 2609, DNA dependent protein kinase catalytic subunit, DNA PK catalytic subunit, DNAPK, DNAPK catalytic subunit, DNPK 1, DNPK1, HYRC 1)

DNA dependent protein kinase (also called DNAPK, DNPK1, HYRC1, protein kinase DNA activated catalytic polypeptide, XRCC7 and p460) consists of the 460kD DNA PKcs and a heterodimeric regulatory complex comprised of p70Ku and p80Ku. DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide. Alternative splicing can occur for this protein to produce at least two isoforms.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:300,000\nWestern Blot: 1:500-1:2000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

168146

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human DNA PKcs. Species Crossreactivity: chimpanzee.

Alternative Names

DNA dependent protein kinase catalytic subunit antibody, DNA PK catalytic subunit antibody, DNAPK antibody, DNAPK catalytic subunit antibody, DNPK 1 antibody, DNPK1 antibody, HYRC 1 antibody