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DNA Polymerase, beta

Cat no: D3935-13C

DNA Polymerase, beta

DNA polymerase beta comprises an amino-terminal 8kD domain and a carboxy-terminal 31kD domain. DNA Polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry (frozen and paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:25-1:50 using ABC method. Incubate for 30 mins. at RT. Formalin fixed, paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining. \n\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Nuclear\n\nPositive Control: Testis\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3935-13C

Size

250ul

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid containing sodium azide

P Type

Mab

Purity

Purified mouse ascites

Isotype

IgG2a

References

i) Husain et al. Nucleic Acids Res 23 (9): 1597, 1995. ii) Srivastava et al. J Biol Chem 270 (27): 16402, 1995.

Additional Info

Recognizes human DNA polymerase beta (Mr 39kD)

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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