DNA polymerase beta is one of the four DNA-directed DNA-polymerases occuring in the nuclei of eukaryotic cells. It is a eukaryotic DNA polymerase primarily involved in gap-filling synthesis during base excision repair. It has unique ability to repair single-stranded DNA gaps smaller than 6 nucleotides and is the only eukaryotic DNA-polymerase able to fill small gaps. It is also responsible for adding new nucleotides to a growing chain by catalyzing a nucleotidyl transfer reaction. It is a single polypeptide chain of 335aa. It has no exonuclease (proofreading) activity.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Optimal dilutions to be determined by the researcher.
Positive Control:
Testis
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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