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DNA Polymerase, beta (POLB)

Cat no: D3935-12B

DNA Polymerase, beta (POLB)

DNA polymerase beta is one of the four DNA-directed DNA-polymerases occuring in the nuclei of eukaryotic cells. It is a eukaryotic DNA polymerase primarily involved in gap-filling synthesis during base excision repair. It has unique ability to repair single-stranded DNA gaps smaller than 6 nucleotides and is the only eukaryotic DNA-polymerase able to fill small gaps. It is also responsible for adding new nucleotides to a growing chain by catalyzing a nucleotidyl transfer reaction. It is a single polypeptide chain of 335aa. It has no exonuclease (proofreading) activity.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nTestis\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D3935-12B

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG2a

References

1. Husain et al. Nucleic Acids Res 23 (9): 1597, 1995. 2. Sobue K and Sellers JR J Biol Chem 266 (19): 12115, 1991.

Additional Info

Recognizes human DNA polymerase beta.

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