DNA Polymerase, epsilon

DNA polymerase e from HeLa cells differs from yeast pol e in that HeLa pol e contains two subunits of 220 and 55kD, whereas yeast pol e has one large subunit and three smaller subunits. Previous studies of template specificity of HeLa pol e, which appears to be required for long patch DNA repair in permeabilized cells, indicate pol e prefers long stretches of single-stranded regions in poly(dA)-oligo(dT) as template. HeLa pol a and pol d prefer\nactivated DNA and alternating poly(dA-dT) as templates, respectively. Pol e retains its processivity in the absence of proliferating cell nuclear antigen (PCNA), unlike pol d. PCNA can also interact with Pol e on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication factor A), and thereby stimulate its primer recognition and DNA synthesis.

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SPECIFICATIONS

Catalog Number

D3935-28

Size

50ul

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse, NHP

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. G. Chui and S. Linn. Further Characterization of HeLa DNA Polymerase e. Journal of Biological Chemistry, 270(14), 7799-7808, 1995. 2. G. Maga et al. Dual Mode of Interaction of DNA Polymerase epsilon with Proliferating Cell Nuclear Antigen in Primer Binding and DNA Synthesis. Journal of Molecular Biology, 285(1): 259-267, 1999.

Additional Info

Specific to the human DNA pol epsilon and does not recognize DNA pol alpha, beta or delta. Species crossreactivity: Can be used for detection of pol epsilon in human, mouse, monkey, mink and hamster.