DNA Polymerase, gamma

Supplier:

United States Biological

Cat no: D3935-35A

DNA Polymerase, gamma

Mitochondria produce cellular ATP through oxidative phosphorylation. Mitochondrial DNA encodes several proteins necessary for oxidative phosphorylation, whereas many other genes are nuclear. DNA Pol gamma (POLG), the only DNA polymerase present in mitochondria, has been implicated in base excision repair process. The catalytic subunit of POLG catalyzes the release of dRP (5' terminal deoxyribose phosphate) from incised apurinic/apyrimidinic sites to produce a substrate for DNA ligase. POLG deficiency has been associated with mtDNA depletion and Alper's syndrome. Low selectivity of mtDNA polymerase is implicated as the cause of many side effects observed in the treatment of viral infections such as HIV.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-5ug/ml. The antibody recognizes >140kD DNA Pol g from HeLa and other cells and tissues.\nELISA: 1:5000-25,000 using 50-100ng control antigen/well.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3935-35A

Size

50ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10 mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

References

1. Longley MJ et al (1998) Biochem. 37, 10529-10539; Muller-Hocker et al (1998) Virchows Arch. 433, 529-536; Davis AF et al (1996) Nucl. Acid Res. 24, 2753-2759; Longley MJ et al (1998) Proc. Natl. Acad. Sci. 95, 12244-12448; naviaux RK et al (1999) Ann Neurol. 45, 54-58

Additional Info

Recognizes human POLG. No crossreactivity is seen with DNA Pol a, b or d. Antibody crossreactivity in various other species has not been studied

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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