Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-780C and remains > 95% active following 1-hour incubation at 950C.
Unit Definition
One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30min at 740C.
Kit Components:
D3947-10A Taq/Pfu DNA Polymerase, 1x250U Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, Stabilizers, 50% glycerol.
D3947-10B Storage Buffer 1x50ul Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, Stabilizers, 50% glycerol.
D3947-10C Reaction Buffer, 10X 1x1ml Supplied as a liquid in 200 mM TrisHCI (pH 8.8) 100 mM KCI 100 mM (NH4)2SO4 20 mM Mg SO4 1% Triton X-100 1 mg/ml BSA.
Note: All reagents, including Taq Plus, should be mixed immediately before use.
Applications:
PCR, 3
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