Kit Components:
D3947-03A: DNA Polymerase, Taq, Thermus aquaticus (Taq) 1x500U Concentration: 50U/ul. Supplied as a liquid in 100mM potassium chloride, pH 8.0, 20mM Tris-HCl, 0.1mM EDTA, 0.5mM PMSF, 50% glycerol, 1mM DTT.
D3947-03B: Buffer 10x Taq buffer 2x1.5ml Supplied as a liquid in 100mM Tris-HCl, (pH 9.0, 500 mM KCl, 15mM MgCl2, 1% Triton X-100
Supplied with a convenient, pre-mixed 10X Reaction Buffer containing the magnesium concentration optimal for most PCR applications.
Applications:
Routine PCR
Preparation of PCR fragments for cloning
Activity:
One unit catalyzes the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 70 degrees C in 50mM Tris-HCl, pH 9.0, 50mM sodium chloride, 5mM MgCl2, 200uM dGTP, dATP, dTTP dCTP (a mix of unlabeled and [33P] dCTP), 10ug of Activated Calf Thymus DNA and 0.1mg/ml BSA.
Absence of Endonuclease or Nicking Activity:
Incubation of 10U of Taq DNA Polymerase with 1ug of supercoiled pBR322 DNA for
16 hours at 70C resulted in no detectable conversion to relaxed or linear forms by agarose
gel electrophoresis.
Absence of Exonuclease Activity:
Incubation of 10U of EconoTaq DNA Polymerase with 1ug of HindIII-cut lambda DNA for
16 hours at 70C resulted in no smearing of bands on agarose gels.
Quality Control:
The enzyme is tested in DNA amplification using a variety of templates and primers.
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