DNA, Salmon Testes, (Protein A Agarose)

Cat no: D3956

Supplier: United States Biological

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Recombinant Protein A covalently bound to agarose by alkylamine linkage. Sonicated salmon sperm DNA blocks non-specific DNA binding sites on protein A agarose when collecting immunocomplexes for chromatin immunoprecipitations (ChIPs assays). Applications: Suitable for use in Chromatin Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by researcher. Quality Control: Immunoprecipitation: 80ul of gel slurry pre-cleared a chromatin solution and subsequently 60ul immunoprecipitated DNA cross-linked to acetylated histones using H5110-13A1: Histone H3, acetylated (Lys9, Lys14). Testing was performed in conjunction with H5110-21: Histone H4, acetylated, Immunoprecipitation Assay Kit, BioAssay(TM). This agarose was also tested with H5110-13Y: Histone H3, acetylated, Immunoprecipitation Assay Kit, BioAssay(TM). Storage and Stability: Stable for 1 year at 4C. Do not freeze.

Catalogue number:	D3956
Applications:	Immunoprecipitation
Size:	1mg
Form:	Supplied as a 50% slurry of agarose beads in 10mM Tris-HCl, 1mM EDTA, pH8, 0.05% sodium azide, 1mg/ml BSA, 3mg/ml Protein A.
References:	1. Mayo, M.W., et al., J. Biol. Chem. 278: 18980-89 (2003). 2. Tomita, A., et al., J. Biol. Chem. 278: 30788-30795 (2003).

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DNA, Salmon Testes, (Protein A Agarose)

Cat no: D3956

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