DNA, ss

Anti-ssDNA (Single-stranded DNA) allows the important discrimination between apoptosis and necrosis. In this respect it can be regarded as a unique research tool superior to TUNEL staining. The specificity of the assay is based upon the high sensitivity of DNA to thermal denaturation in condensed chromatin of apoptotic cells. Although there is no change of the original product there have been important improvements in the staining protocol involving a formamide-based DNA-denaturation. This is now more convenient to use, yielding a sharper contrast between apoptotic cells and controls. \n\nApplications: \nSuitable for use in Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry: Frozen and paraffin sections\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D3878-16B

Size

200ul

Applications

ELISA, FC, ICC, IF, IHC

Hosts

Mouse

Reactivities

Bov

Form

Supplied as a liquid in PBS, 5% fetal bovine serum (FBS).

P Type

Mab

Purity

Purified

Isotype

IgM

References

[1] O.S. Frankfurt; Exp. Cell. Res. 170, 369 (1987) [2] O.S. Frankfurt; Anticancer Res. 14, 1861 (1994) \n[3] O.S. Frankfurt; Exp. Cell. Res. 191, 181 (1990) [4] Neuroreport 7, 61 (1995) [5] B. Grasl-Kraupp, et al.; Hepatology 21, 1465 (1995) [6] O.S. Frankfurt, et al.; Exp. Cell. Res. 226, 387 (1996) [7] O.S. Frankfurt; (Eds L. Zhu & J. Chun) 47 (1998) [8] O.S. Frankfurt & A. Krishan; J. Histochem. Cytochem. 49, 369 (2001) [9] P. Bu, et al.; J. Immunol. 166, 6399 (2001) [10] M. Zurita, et al.; J. Neurosurg. 96, 83 (2002) [11] J. Vaquero, et al.; Acta Neurochir. (Wien) 144, 151 (2002) [12] S.P. Aries, et al.; Scand. J. Immunol. 48, 86 (1998) [13] E. Adamec, et al.; Brain Res. 849, 67 (1999) [14] M. Provinciali, et al.; Life Sci. 64, 1525 (1999) [15] J.C. Wasmuth, et al.; J. Acquir. Immune Defic. Syndr. 23, 44 (2000) \n[16] M. C. Michallet, et al.; Blood 102, 3719 (2003)

Additional Info

Recognizes bovine deoxycytidine of ssDNA of at least 25-30 bases in length in the absence of any reactivity to double-stranded DNA. Not species-specific. Specifically detects apoptotic cells. Necrotic cells are not recognized.