DNA, ss

Anti-ssDNA (Single-stranded DNA) allows the important discrimination between apoptosis and necrosis. In this respect it can be regarded as a unique research tool superior to TUNEL staining. \n\nApplications:\nSuitable for use in ELISA and Farr Assay. Other applications have not been tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nBiological Activity:\nFarr Assay: Demonstrates anti-ssDNA activity.\nNote: 24% (normal <13%) of the radio-iodinated DNA precipitates with this antibody.\n\nAutoantibody Activity:\nAnti-ssDNA Activity by ELISA: Current lot value: 397IU/ml\nInterpretation of Results: <16 EU/ml: Negative; 16-20EU/ml: Equivocal; Positive; >20 EU/ml:\nSSA: 129 EU/ml\nSSB, SCL, Sm: 0 EU/ml \nSm/RNP, Jo-1: 1 EU/ml\ndsDNA: 32 EU/ml\n\nAnti-ssDNA Activity by ELISA: As reported (lot-specific). Current lot value: 397U/ml\nInterpretation of Results: <68.6 U/ml: Negative; >68.6U/ml: Positive\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.пїЅ

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SPECIFICATIONS

Catalog Number

D4015-05

Size

2ml

Applications

ELISA

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.

P Type

Pab

Purity

Purified by fractionation of delipidated and defibrinated donor human serum.

Isotype

IgG

Additional Info

Displays only Anti-ssDNA activity when tested by ELISA. Undiluted antibody shows a strong Scl-70 precipitin band. Specificity verified by a precipitin line of identity with the CDC-AF reference serum and by ELISA procedures using both native DNA and ssDNA. Does not crossreact at a dilution of 1:100 when tested against purified Ro/SS-A, La/SS-B, Sm, Scl-70, nRNP, dsDNA, or Jo-1 antigens in an ELISA system designed to detect IgG