DNA topoisomerase II, CT (TOP2A, TOP2, DNA topoisomerase 2-alpha isozyme) (FITC)

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze FITC conjugates. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

034689-FITC

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to FITC.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Vranic, S., et al. Hum. Pathol. 41(11):1617-1623(2010)\nChen, H., et al. Am. J. Surg. Pathol. 34(9):1250-1257(2010)\nYe, J., et al. Cell 142(2):230-242(2010)\nRossi, E., et al. Histopathology 57(1):81-89(2010)\nSugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

Additional Info

Human

Alternative Names

TOP2A, TOP2, DNA topoisomerase 2-alpha isozyme