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DnaK, Recombinant, E. coli

Cat no: D4015-11

DnaK, Recombinant, E. coli

DnaK is an acidic 70kD Escherichia coli heat shock protein which belongs to the molecular chaperone class of proteins (1). The sequence of the dnaK gene is highly homologous with eukaryotic Hsp70 proteins. Reversible binding of Hsp70 to unfolded nascent polypeptides, partially denatured proteins and also some native proteins leads to: i) the stabilization of polypeptide chains during de novo folding, ii) protection of other proteins from aggregation, iii) reactivation of heat inactivated enzymes, iv) promotion of peptide translocation through membranes, and v) exposure of partially denatured proteins for efficient proteolysis. The N-terminal domains of Hsp70 proteins contain an ATPase active site, and the C-terminal domains contain a peptide binding site. DnaK has been shown to bind to various aberrant proteins including protein fragments, mutant proteins, unfolded proteins as well as native proteins: lambdaP, lambdaO and sigma32. DnaK

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SPECIFICATIONS

Catalog Number

D4015-11

Size

50ug

Applications

WB

Form

Supplied as a liquid in 40mM Tris, pH 7.5, 80mM sodium chloride, 0.8mM DTT, 0.08mM PMSF, 20% glycerol.

Purity

(same/more than) 95% as determined by SDS-PAGE analysis.

References

1. Zylicz, M., LeBowitz, J. H., McMacken, R., Georgopoulos, C. (1983) PNAS USA, 80: 6431-6435.

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

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Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

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Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

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Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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