Dnmt1, phosphorylated (Ser714) (DNA (cytosine-5)-methyltransferase 1, Dnmt1, MCMT, DNA Methyltransferase HsaI, DNA MTase HsaI, M.HsaI, CXXC-type Zinc Finger Protein 9, AIM, CXXC9, DNMT) (PE)

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.\n\nApplications: \nSuitable for use in ELISA and Dot Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000 \nDot Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze R-Phycoerythrin conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D3931-63E-PE

Size

200ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to R-Phycoerythrin (R-PE).

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1.Peterson, E.J., et al., Cancer Res. 63(20):6579-6582 (2003). 2.Leu, Y.W., et al., Cancer Res. 63(19):6110-6115 (2003). 3.Saito, Y., et al., Int. J. Cancer 105(4):527-532 (2003). 4.Siedlecki, P., et al., Biochem. Biophys. Res. Commun. 306(2):558-563 (2003). 5.Macaluso, M., et al., Oncogene 22(23):3511-3517 (2003).

Additional Info

Recognizes human Dnmt1 when phosphorylated at Ser714.