Dnmt3a (DNA (Cytosine-5)-methyltransferase 3A, DNA Methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA, DNMT3A)

Dnmt3a, also known as DNA (cytosine-5)-methyltransferase 3A, is a nuclear protein belonging to the C5-methyltransferase family with an ADD-type zinc finger and a PWWP domain. This nuclear protein localizes to euchromatin and is essential for genome wide de novo methylation and is also essential for development. DNA methylation is coordinated with methylation of histones. Dnmt3a associates with HDAC1 through its ADD-type zinc-finger. It binds to the ZNF238 transcriptional repressor and is also known to interact with other DNA methyltransferases like Dnmt1 and Dnmt3b.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nDnmt3a transfected cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D3931-70S

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Chen, T. et al. J. Biol. Chem. 277:38746-38754 (2002). 2. Schlesinger, Y. et al. Nat. Genet. 39:232-236 (2007). 3. Li H. et al. J. Biol. Chem. 281:19489-19500 (2006).

Additional Info

Recognizes human Dnmt3a. Species Crossreactivity: chimpanzee, rhesus monkey. Species sequence homology: feline, bovine, equine, mouse, rat.