DOK1, CT (Docking Protein 1 62kD, Downstream Of Tyrosine Kinase 1, MGC117395, MGC138860, p62(dok), P62DOK, pp62)

Docking proteins interact with receptor tyrosine kinase to mediate particular biological responses. DOK1 is a major substrate for a range of tyrosine kinases including c-kit, v-abl, v-Fms, v-Src along with activated EGF, PDGF, IGF, VEGF and insulin receptors. \n\nResearch indicates that DOK1 is a negative regulator of the insulin signalling pathway. \n\nDok1 is expressed in a range of human tissues including the pancreas, heart and spleen, it is also expressed by both resting and activated peripheral blood T-cells.\n\nApplications:\nSuitable for use in Immunohistochemistry (frozen) and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nImmunohistochemistry (frozen): 2ug/ml\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D8080-01J

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Carpino N., et al (1997) p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Cell 88:197-204. 2. Yamanashi Y., et al (1997) Identification of the Abl- and rasGAP-associated 62 kDa protein as a docking protein, Dok. Cell \n88:205- 211. 3. Holland S.J., et al (1997) Juxtamembrane tyrosine residues couple the Eph family receptor EphB2/Nuk to specific SH2 domain proteins in neuronal cells. EMBO J 16:3877-3888. 4. Di Cristofano A, et al (1998) Molecular cloning and characterization of p56(dok-2) defines a new family of RasGAP-binding proteins. J. Biol. Chem. 273:4827-4830.

Additional Info

Recognizes human DOK1 (Downstrean of tyrosine kinase).