Dok5 (Docking Protein 5, C20orf180, dJ805C22.1, Downstream of Tyrosine Kinase 5, IRS6, MGC16926, Protein Dok-5)

The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. In contrast to other DOK family proteins, this protein does not interact with RASGAP.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:16,000\nWestern Blot: 1-3ug/ml In transfected HEK293 transiently expressing DOK5 a band of ~38kD is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 35.5kD according to NP_060901.2.\nImmunohistochemistry: 1-2ug/ml In paraffin embedded tonsil, stains scattered cells in the interfollicular area compatible with mast cells.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D8080-12B

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1.Cai D, Dhe-Paganon S, Melendez PA, Lee J, Shoelson SE. Two new substrates in insulin signaling, IRS5/DOK4 and IRS6/DOK5.J Biol Chem. 2003 Jul 11;278(28):25323-30. Epub 2003 May 01.

Additional Info

Recognizes human Dok5. Species sequence homology: mouse, rat, canine.

Alternative Names

chromosome 20 open reading frame 180, downstream of tyrosine kinase 5, docking protein 5, C20orf180, MGC16926, DOK5