Dopamine Beta Hydroxylase (DBH, Dopamine beta Monooxygenase)

The production of neurotransmitters is controlled by environmental stimuli, which send signals through second messengers and protein kinase pathways. Regulation of gene expression of dopamine beta hydroxylase and tyrosine hydroxylase, two of the catecholamine biosynthetic enzymes, by cyclic AMP, diacyl glycerol, and Ca2+ leads to increased neurotransmitter biosynthesis. Dopamine beta-hydroxylase catalyzes the conversion of dopamine to noradrenaline and is expressed selectively in noradrenergic and adrenergic neurons in the nervous system. The disruption of the DBH gene is thought to cause changes in development and behavior in certain models. Both DBH and the multiple regulatory pathways of DBH are of increasing research in many neurological diseases.\n\nApplications: \nSuitable for use in RIA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry: 1:2000 on rabbit and guinea pig using an ABC detection\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9010-06

Size

50ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat, Bov

Form

Supplied as a lyohilized powder. No preservatives. Reconstitute with 50ul sterile ddH2O.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Rush, R. and Geffen L. (1980) Crit Rev Clin Lab Sci, 12:241-277 (1980)

Additional Info

Recognizes bovine Dopamine beta hydroxylase (DBH). Reacts with a single band on Western Blots of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with enzyme. Species Crossreactivity: human and rat