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Dopamine Beta Hydroxylase (DBH, Dopamine beta Monooxygenase)

Dopamine Beta Hydroxylase (DBH, Dopamine beta Monooxygenase)

Cat no: D9010-06A

Supplier: United States Biological
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Dopamine beta-hydroxylase (DbetaH; also dopamine beta-monooxygenase) is a 73-77kD member of the copper type II ascorbate-dependent monooxygenase family. It is both soluble (73kD) and membrane-bound (77kD) (anchored by an uncleaved signal sequence), and via hydroxylation, converts dopamine into norepinepherine. Human DbetaH is a copper-containing disulfide-linked homodimer that is found in neurons and adrenal medullary cells. It is 603aa in length and contains a 25aa signal sequence followed by three domains. The first is an N-terminal 120aa DOMON domain (dopamine beta-monooxygenase N-terminal) that may either bind DbetaH to the cell membrane, or participate in tetramerization. This is followed by two 150aa Cu + -type II ascorbate-dependent monooxygenase domains (aa182-330 and 352-512). DbetaH may be most active as a dimeric-dimer/tetramer, whose association status is dependent on local Cl-concentrations. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Sufficient for 10 mini-blots. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D9010-06A
Reactivities: Human, Non-Human Primate
Hosts: Sheep
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Kreek, M.J. et al. (2005) Pharmacol. Rev. 57:1. 2. Stewart, L. and J.P. Klinman (1999) FEBS Lett. 454:229. 3. Houhou, L. et al. (1995) J. Biol. Chem. 270:12601. 4. Timmers, H. et al. (2004) Ann. N.Y. Acad. Sci. 1018:520. 5. Lamouroux, A. et al. (1987) EMBO J. 6:3931.
Additional info: Specific for the ~75kD DbH protein in Western blots of human adrenal medulla. Species Cross Reactivity: Monkey, mouse.

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