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Dopamine Beta Hydroxylase, Native (Dopamine beta-hydroxylase, DBH, Dopamine beta Monooxygenase, DBM)

Dopamine Beta Hydroxylase, Native (Dopamine beta-hydroxylase, DBH, Dopamine beta Monooxygenase, DBM)

Cat no: D9010-03B

Supplier: United States Biological
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DBH is an oxireductase belonging to the copper type II ascorbate-dependent monooxygenase family. DBH exists as a homotetramer composed of two non-covalently bound disulfide-linked dimers. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It binds 2 copper ions and 1 PQQ per subunit . Depending on the presence of a signal peptide, DBH can exist in both soluble and membrane-bound forms. Applications: Suitable for use in Western Blot, Immunohistochemistry, radioimmunoassay and inhibition of enzymatic activity.. Other applications not tested. Recommended Dilution: Western Blot: 1-500-1:2000 Immunohistochemistry: 1-500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D9010-03B
Reactivities: Human, Rat, Bovine, Guinea Pig
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a lyophilized powder. Reconstitute with 100ul sterile dH2O.
P type: Pab
Purity: Serum
Alternative names: EC=1.14.17.1
References: 1. Rush R. A. et al (1980) Gut Rev clin Lab Sci 12: 241, 2. Verndell I. M. et al (1982) J Histochem Cytochem 30: 682.
Additional info: Recognizes bovine Hydroxylase (TH). Species Crossreactivity: human, guinea pig, rat.

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