Dopamine (BSA)

Dopamine (C6H3(OH)2-CH2-CH2-NH2) is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.\n\nApplications: \nSuitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 6 months at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% merthiolate. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9000-01C

Size

1mg

Applications

ICC, IHC

Form

Supplied as a lyophilized powder. Conjugated to BSA via a glutaraldehyde linker. Reconstitute with sterile ddH2O, 0.1% merthiolate.

Purity

Purified

References

US Biological application references: 1. Kim, J. et al., J. Immunoassay and Immunochemistry (2010) 31: 33-44. 2. Kim, J. et al., (2008) Analytica Chimica Acta 619:87-93 (ELISA). 1.Chagnauld, J.L., et al. J. Neurochem. (1987) 49: 487-494. 2Decavel, C., et al. First visualization of dopaminergic neurons with a monoclonal antibody to dopamine: a light and electron microscopic study. J. Histochem. Cytochem. 35: 1245-1252 (1987). 3.Schurmann, F.W.,, et al. Cell & Tissue Res. 256: 399-410 (1989). 4. Goldman-Rakic, P.C., et al. Proc. Nat. Acad. Sci. USA 86: 9015-9019 (1989).