Dopamine Receptor, D1, aa9-21

Applications: \nSuitable for use in Immunocytochemical and Western Blotting detection of the receptor. \n\nRecommended Dilution:\nFound to stain specific cells in various regions of PLP fixed rat brain sections at a dilution of 1:5000. Includes the Medial septum, Nuclcus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area.\n\nWestern Blot: Using whole rat brain homogenate resulted in a single band being detected at ~50 Kd at 1:500 dilution.\nOptimal dilution determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume (100ul) by adding sterile 25% glycerol in PBS with 10mg/ml BSA and store at -20 degrees C. May be stored at 4 degrees C for short-term only. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9000-05

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Lyophilized. Rehydrate with 0.1ml of PBS, 10mg/ml BSA.

P Type

Pab

Purity

Purified

Isotype

IgG

Additional Info

Recognizes rat Dopamine Receptor, D21 Highly specific for this peptide sequence and are suitable for immunocytochemical and western blot detection of the D1 dopamine receptor. Polypeptide % Crossreactivity D1Dopamine Receptor (9-21) 100 D1Dopamine Receptor 90 D2Dopamine Receptor (272-282) 0 D2Dopamine Receptor 0 D3Dopamine Receptor (2-10) 0 D3Dopamine Receptor 0 D4Dopamine Receptor (176-185) 0 D4Dopamine Receptor 0 D5Dopamine Receptor (23-35) 0 D5Dopamine Receptor 0