Dopamine Receptor D1 (DRD1)

Dopamine Receptor D1 (DRD1) belongs to the G-protein coupled receptor 1 family and it is one of the five types of dopamine receptors (D1-D5). DRD1 is the most expressed dopamine receptor in central nervous system and it is involved in the regulation of neuronal growth and development, and modulation of dopamine receptor D2-mediated events. G protein coupled DRD1 activates adenylyl cyclase which leads to activation of cyclic AMP dependent protein kinases. Polymorphism of DRD1 gene has been implicated in bipolar I disorder and attention deficit hyperactivity disorder (ADHD).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 40ug/ml detected DRD1 at ~50kD using whole rat brain homogenate.\nImmunocytochemistry: 5ug/ml stains cells in various regions of PLP fixed rat brain sections. These regions include the Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantis Nigra reticulata and the Ventral tegmental area.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D9000-04F

Size

50ul

Applications

ELISA, ICC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS. Dilute further in PBS, 10mg/ml BSA.

P Type

Pab

Purity

Purified

Isotype

IgG

Additional Info

Recognizes the Dopamine Receptor D1 (DRD1). Reactivity with DRD1 (aa9-21) is 100% and 90% with full length DRD1. Does not crossreact with D2, D3, D4 or D5 Dopamine Receptors. Species Crossreactivity: Rat