Dopamine Receptor D2 (DRD2)

The D2 dopamine receptor is one of five (D1 to D5) receptors for dopamine. Its activity is mediated by G proteins which inhibit adenylyl cyclase. It interacts with GPRASP1, neurabin-2, CLIC6, CADPS and CADPS2. It is a transmembrane protein that belongs to the G-protein coupled receptor 1 family. The unprocessed precursor has a length of 443 amino acids and an estimated molecular mass of 50.6kD. Genetic variations in DRD2 may determine the genetic susceptibility to alcoholism. A missense mutation in this gene causes myoclonus dystonia and several other defects in this gene are thought to be involved in psychiatric disorders, especially in schizophrenia.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nDilute (same/more than)1:8 in 1% normal goat serum, TBS/Tween-20 before use. Optimal dilutions to be determined by the researcher.\n\nIncluded Control Peptide:\nD9000-06L1: Dopamine Receptor D2 Control Peptide (DRD2)\nReconstitute with 600ul of reconstituted antibody.\n\nPeptide Amino Acid Sequence (Cys271-272-282) : \nNH2-Cys-Ala-Ala-Arg-Arg-Ala-Gln-Glu-Leu-Glu-Met-Glu-COOH\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9000-06L

Size

5ml

Applications

ELISA, ICC, WB

Hosts

Rabbit

Form

Supplied as a lyophilized powder. Reconstitute with 5ml PBS, 10mg/ml BSA.

P Type

Pab

Purity

Purified

Isotype

IgG

Additional Info

Recognizes the D2 Dopamine Receptor (DRD2). Reactivity with DRD2 (aa272-282) is 100% and 60% for full-length DRD2. Detects both the D2S and D2L Dopamine Receptors in Western Blot. Does not crossreact with the D1, D3, D4 or D5 Dopamine Receptors.