Dopamine Receptor, D2R

Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L)forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29 amino acid in the intracellular, cytoplasmic loop 3. D2L receptor is a 446 aa, G- protein coupled, and transmembrane receptor protein. D2R may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:5000 (ECL)\nELISA: 1:10,000-1:50,000 Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: \nD9016-42: Dopamine Receptor, Rat, Control Peptide, D2R\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D9016-40

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, 0.05% sodum azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Bunzow, JR (1989) Nature 336, 783-7871. 2. Sakata, M et al (1992) Brain Res. 575, 309-314. 3. Farooqui, SM (1991) J Neurochem. 57, 1363-1369. 4. Farooqui, SM et al (1992) BBRC 184, 661-667. 5. Brock, J et al (1992) Brain Res. (1992) 578, 244-250. 6. Farooqui, SM et al (1992) Life. Sci.

Additional Info

Recognizes rat Dopamine Receptor, D2R. Species Crossreactivity: human (CNS and peripheral), lobster, squid and crawfish.