Dopamine Receptor, D2s, aa239-246

The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. 2 isoforms are reported for the D2 Dopamine Receptor, Isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 40ug/ml using whole rat brain homogenate resulted in a single band being detected at ~48kD.\nImmunohistochemistry/Immunocytochemistry: 80ug/ml stains specific cells in various regions of PLP fixed rat brain sections including the Medial septum, Nuclcus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra teticulata and the Ventral regmental area.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9000-13

Size

100ul

Applications

ELISA, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a lyophilized powder from 100mM ammonuim bicarbonate. Reconstitute with 100ul sterile PBS, 10mg/ml BSA.

P Type

Pab

Purity

Purified by precipitation, dialysis and chromatography.

Isotype

IgG

Additional Info

Recognizes Dopamine Receptor, D2s, aa239-246. Species Crossreactivity: rat. Crossreacts with Dopamine Receptor, D2s (50%). Does not react with D2L Dopamine Receptor, D1 Dopamine Receptor (9-21), D1 Dopamine Receptor, D3 Dopamine Receptor (2-10), D3 Dopamine Receptor, D4 Dopamine Receptor (176-185), D4 Dopamine Receptor, D5 Dopamine Receptor (23-35), D5 Dopamine Receptor.