Dopamine Transporter (DAT)

Dopamine is an endogenous catecholamine that influences many cellular activities. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based on the aa identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B and D5) or D2-like (D2, D3 and D4). The two isoforms of D2R, D2 long (D2L) and short (D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions. Dopamine transporter (DAT) is plasma membrane transporter for the termination of dopaminergic neurotransmission by its high affinity Na+ and Cl- reuptake of dopamine into presynaptic terminals. This protein is the target of psychomotor stimulants such as amphetamines or cocaine. DAT (human 620aa; rat/mouse 619aa glycoprotein) is predicted to contain 12 transmembrane domains with N- and C-termini in the cytoplasm.\n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:5000 (ECL)\nELISA: 1:10,000-1:50,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D9020-18

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Kilty, J.E., et al., Science 254: 578-579 (1991). 2. Giros, B., et al., FEBS Lett. 295: 149-154 (1991). 3. Shimada, S., et al., Science 254: 576-578 (1991). 4. Kitayama, S., et al., PNAS 89: 7782-7785 (1992). 5. Freed, C., et al., J. Comp. Neurology 359: 340-349 (1995). 6. Vaughn, R.A., et al., J. Neurochem. 66: 2146-2152 (1996). 7. Lew, R., et al., Brain Res. 584: 2666-271 (1992).

Additional Info

Recognizes human Dopamine Transporter (DAT). No significant sequence homology is seen with other transporters.