DOT1L (DOT1-like, the mammalian homologue of yeast DOT1), is a DNA-binding protein that acts as a histone methyltransferase. DOT1L di- and trimethylates histone H3 on lysine 79, and is thought to be the sole enzyme responsible for this mark. DOT1L and H3K79 methylation play important roles in heterochromatin formation and in embryonic development. In contrast to other lysine histone methyltransferases, DOT1L does not contain a SET domain, suggesting a unique mechanism for methylation of lysines in histones DOT1L is associated with activating genes associated with acute leukemia by deregulation of cancer-relevant genes and binding with several MLL fusion partners. In yeast, Dot1-mediated H3K79 methylation is associated with telomere silencing, meiotic checkpoint control, and DNA damage response.
Applications:
Suitable for use in ELISA and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:1000
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quick Search
Products 
