DOT1L (KMT4, Histone-lysine N-methyltransferase, H3 Lysine-79 Specific, Histone H3-K79 Methyltransferase, H3-K79-HMTase, DOT1-like Protein, Lysine N-methyltransferase 4, KIAA1814)

DOT1L is an evolutionarily conserved histone methyltransferase which methylates lysine 79 of histone H3 in the core domain. Unlike other histone methyltransferases, the protein does not contain a SET domain, an evolutionary conserved motif, and it specifically methylates nucleosomal histone H3. The protein was originally identified in S. cerevisiae as a disruptor of telomeric silencing. Analysis of the protein sequence revealed no known functional motif other than a putative SAM binding domain. Recent reports also suggests that the protein interacts with AF10, a mixed lineage leukemia fusion partner involved in acute myeloid leukemia, which results in leukemic transformation in a DOT1L methyltransferase activity-dependent manner. Human DOT1L gene is localized in the chromosomal region 19p13.3.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:100-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D9056-01F

Size

100ug

Applications

ELISA

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Jinrong, M. et al. Cell. 112:711-723 (2003). 2. Okada, Y. et al. Cell. 121:809-811 (2005). 3. Feng, Q. et al. Current Biology. 12:1052-1058 (2002).

Additional Info

Recognizes human DOT1L. Species sequence homology: mouse.