DPF2 (REQ, UBID4, ubi-d4, MGC10180, Zinc Finger Protein ubi-d4, Protein Requiem, Apoptosis Response Zinc Finger Protein, D4, Zinc And Double PHD Fingers Family 2, BRG1-associated Factor 45D, BAF45D)

Requiem is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It is expressed in numerous tissues in both cytosolic and nuclear regions and likely serves a regulatory role in rapid hematopoietic cell growth and turnover. Requiem has been found to act as an adaptor protein that links the NF-kB and SWI/SNF chromatin remodeling factor and is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse Kidney Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146779

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Gabig TG, Mantel PL, Rosli R, et al. Requiem: a novel zinc finger gene essential for apoptosis in myeloid cells. J. Biol. Chem.1994; 269:29515-9. 2. Gabig TG, Crean CD, Klenk A, et al. Expression and chromosomal localization of the Requiem gene. Mamm. Genome1998; 9:660-5. 3. Tando T, Ishizaka A, Watanable H, et al. Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway. J. Biol. Chem.2010; 285:21951-60.

Additional Info

Recognizes human Requiem. Species Crossreactivity: mouse.