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DPPA2 (Developmental Pluripotency-associated Protein 2, Pluripotent Embryonic Stem Cell-related Gene 1 Protein, PESCRG1)

Cat no: D3221-94G

DPPA2 (Developmental Pluripotency-associated Protein 2, Pluripotent Embryonic Stem Cell-related Gene 1 Protein, PESCRG1)

DPPA2 is a 38-39kD member of both the DPPA and cancer-testis family of proteins. It is found in pluripotent stem cells in a pattern that parallels that of Oct4, and likely contributes to formation of the ICM, plus the maintainence of cells in an undifferentiated state. DPPA2 is also found in spermatocytes and select tumor types. Human DPPA2 is 298aa in length. It contains one DNA-binding SAP domain that is involved in chromosomal organization aa92-126. One potential isoform variant has been reported that shows a deletion of aa61-71. Over aa2-174, human DPPA2 shares only 36% aa identity with mouse DPPA2.\n\nApplications:\nSuitable for use in Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry: 5-15ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3221-94G

Size

100ug

Applications

ICC

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human DPPA2.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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