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DPT (Dermatopontin, Tyrosine-rich Acidic Matrix Protein, TRAMP)

DPT (Dermatopontin, Tyrosine-rich Acidic Matrix Protein, TRAMP)

Cat no: D3212-51C

Supplier: United States Biological
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Dermatopontin, is a widely expressed noncollagenous protein component of the extracellular matrix (1,2). Mature human Dermatopontin shares 96%, 92%, and 92% aa sequence identity with bovine, mouse, and rat Dermatopontin, respectively. It is a 22kD molecule that is tyrosine sulfated but not glycosylated (3,4). Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5). It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6-8). Dermatopontin is downregulated in fibrotic growths such as leiomyoma and scar tissue (9,10). It binds both TGF-b and the proteoglycan decorin, interactions that can increase the bioavailability of TGF-b (11,12). Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13,14). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D3212-51C
Reactivities: Human, Mouse
Hosts: Sheep
Size: 100ug
Form: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Okamoto, O. and S. Fujiwara (2006) Connect. Tissue Res. 47:177. 2. Superti-Furga, A. et al. (1993) Genomics 17:463. 3. Forbes, E.G. et al. (1994) FEBS Lett. 351:433. 4. Cronshaw, A.D. et al. (1993) Matrix 13:255. 5. Neame, P.J. et al. (1989) J. Biol. Chem. 264:5474. 6. MacBeath, J.R.E. et al. (1993) J. Biol. Chem. 268:19826. 7. Takeda, U. et al. (2002) J. Invest. Dermatol. 119:678. 8. Cooper, L.J. et al. (2006) Invest. Opthalmol. Vis. Sci. 47:3303. 9. Catherino, W.H. et al. (2004) Genes Chromosomes Cancer 40:204. 10. Kuroda, K. et al. (1999) J. Invest. Dermatol. 112:706. 11. Okamoto, O. et al. (1996) J. Biochem. 119:106. 12. Okamoto, O. et al. (1999) Biochem. J. 337:537. 13. Pochampally, R.R. et al. (2007) J. Bone Miner. Res. 22:1338. 14. Behnam, K. et al. (2006) Connect. Tissue Res. 47:271.
Additional info: Recognizes human Dermatopontin. Species sequence homology: Recombinant mouse Dermatopontin (50%).

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