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DR1, aa1-176, Recombinant, Human (Down-regulator of transcription 1, TBP-binding)

Cat no: D9070-04

DR1, aa1-176, Recombinant, Human (Down-regulator of transcription 1, TBP-binding)

DR1, also known as NC2-beta, is a TBP (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 may shift the physiological balance of transcriptional output in favor of polymerase I.\n\nSource:\nRecombinant corresponding to aa1-176 of human recombinant DR1 protein, fused to His-tag at N-terminus, expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MASSSGNDDD LTIPRAAINK MIKETLPNVR VANDARELVV NCCTEFIHLI SSEANEICNK SEKKTISPEH\nVIQALESLGF GSYISEVKEV LQECKTVALK RRKASSRLEN LGIPEEELLR QQQELFAKAR QQQAELAQQE WLQMQQAAQQ AQLAAASASA \nSNQAGSSQDE EDDDDI\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D9070-04

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.1mM PMSF, 10% glycerol.

Purity

~85% (SDS-PAGE)

References

1. White RJ., et al. (2010) Nucleic Acids Res. 38(4):1228-39. 2. Collart MA., et al. (2008) Nucleic Acids Res. 36(2):539-49.

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