DR4, CT (TRAIL R1, TRAIL Receptor1, Death Receptor4)

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4)(1). The ligand for this novel death receptor has been identified and termed TRAIL(2), which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1)(3). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NFkB activation in many tissues and cells. \n\nApplications: \nSuitable for the detection of DR4 expression by Western blot(4,5). Whole cell lysate from HeLa cells can be used as positive control and a 57kD band should be detected. Western blot analysis of 50ug of total cell lysate from HeLa cells with anti-DR4 (CT) at 1:500 dilution. \n\nRecommended Dilution: \nWestern blot: 1:500-1:1000\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9300-32A

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Pan G; et al., Science; 1997; 276:111-113. 2. Pitti RM; et al., J. Biol. Chem. 1996; 271:12687-90. 3. Schneider P, et al.,. Immunity 1997; 7:831-836. 4. Manal A. Eid, et al., Mol. Cancer Ther. 1: 831-840 (2002). 5. John R. Basile, et al,. J. Biol. Chem, 276: 22522

Additional Info

Recognizes human DR4, CT (TRAIL R1, Death Receptor4)