DR5, Blocking Peptide (Apo2, Trail-R2, TRAIL Receptor2, Trick2, Killer, Death Receptor5, CD262, KILLER, KILLER/DR5, TNFRSF10B, TRANCE-R, TRICKB, Tumor Necrosis Factor Receptor Superfamily, Member 10b, ZTNFR9)

Peptide corresponding to amino acids 388 to 407 of human DR5 precursor (1,2).\n\nApoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-7). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB. Human sTRAIL Receptor-2 is a 14.9kD protein containing 133 amino acid residues comprising the extracellular domain of TRAIL Receptor-2. \n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Other applications not tested.\n\nRecommended Dilution:\nIncubation of peptide with equal volume of antibody for 30 minutes at 37 degrees C completely blocks the antibody activity in Westen Blot.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9300-50P

Size

50ug

Form

Supplied as a liquid in PBS, pH7.2, 0.1% BSA, 0.02% sodium azide.

References

1. Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 277:815-8 (1997) 2. Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science ;277:818-21 (1997).3. Walczak H, et al.,TRAIL. EMBO J 1997;16:5386-97 4. MacFarlane M, et al., J Biol Chem 1997;272:25417 5. Screaton GR, et al., Curr Biol 1997;7:693-6 6. Wu GS, et al., Nat Genet 1997;17:141-3 7. Chaudhary PM, et al., Immunity ;7:821-30 (1997).