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DRAK1 (DAP Kinase-related Apoptosis-inducing Protein Kinase 1, Death-associated Protein Kinase-related 1, Serine/threonine Kinase 17a Death Associated Protein Kinase Related 1, STK17A)

Cat no: D9334-02B

DRAK1 (DAP Kinase-related Apoptosis-inducing Protein Kinase 1, Death-associated Protein Kinase-related 1, Serine/threonine Kinase 17a Death Associated Protein Kinase Related 1, STK17A)

DRAK1 (DAP kinase-related apoptosis-inducing protein kinase 1; also STK17A is a 55kD member of the DAP kinase subfamily, CAMK Ser/Thr protein kinase family of enzymes. It is expressed in osteoclasts, resides in the nucleus, and initiates apoptosis. Human DRAK1 is 414aa in length. It contains an N-terminal polyPro segment aa33-37 followed by a Ser/Thr kinase domain aa64-321 with a catalytic loop aa84-193. Apoptosis apparently requires an intact kinase domain. No rodent DRAK1 has been reported. Over aa115-313, human DRAK1 shares 95% aa identity with canine DRAK1, and 65%aa identity with human DRAK2.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9334-02B

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human DRAK1. No cross-reactivity with recombinant human DRAK2, DAPK1, DAPK2, or DAPK3.

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