DRAK2, CT (DAP Kinase-related Apoptosis-inducing Protein Kinase 2)

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain an N-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues (1).\n\nThis polyclonal antibody can be used for detection of DRAK2 by Western blot at 1:500 to 1:1000 dilution. Whole cell lysate from Jurkat cells can be used as positive control and an approximately 45kD band can be detected. It has no cross responses to DAP or ZIP kinases. The approximately 70kD band is probably non-related to DRAK2 although it is peptide blockable. \n\nWestern blot analysis of DRAK2 in Jurkat (1,3) and Raji (2,4) cell lysate in the absence (1,2) or presence (3,4) of blocking peptide with anti-DRAK2 (CT) at 1:500 dilution.\n\n

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SPECIFICATIONS

Catalog Number

D9335

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified IgG

Isotype

IgG

References

1. Sanjo H, Kawai T, Akira S. DRAKs, novel serine/threonine kinases related to death-associated protein kinase that trigger apoptosis. J Biol Chem 1998;273:29066-71

Additional Info

The approximately 70kD band is probably non-related to DRAK2 although it is peptide blockable.