Draxin (Dorsal repulsive axon guidance protein, neucrin) is a secreted, 58kD, presumable glycoprotein member of the Draxin family of molecules. In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite. Draxin acts as a Wnt antagonist, apparently by binding to LRP6. The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation). Mature mouse Draxin is 318aa in length. It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa268-327). Mature mouse Draxin (aa26-343) shares 80% aa identity with mature human Draxin, and 94% aa identity with mature rat Draxin.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.5ng/well.
Western Blot:1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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