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Draxin (Dorsal Repulsive Axon Guidance Protein, Neucrin)

Cat no: D9392-01

Draxin (Dorsal Repulsive Axon Guidance Protein, Neucrin)

Draxin (Dorsal repulsive axon guidance protein, neucrin) is a secreted, 58kD, presumable glycoprotein member of the Draxin family of molecules. In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite. Draxin acts as a Wnt antagonist, apparently by binding to LRP6. The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation). Mature mouse Draxin is 318aa in length. It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa268-327). Mature mouse Draxin (aa26-343) shares 80% aa identity with mature human Draxin, and 94% aa identity with mature rat Draxin.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nDirect ELISA: 0.5-1ug/ml. The detection limit is ~0.5ng/well.\nWestern Blot:1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9392-01

Size

100ug

Applications

ELISA, WB

Hosts

Sheep

Reactivities

Mouse, Rat

Form

Supplied as lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes mouse Draxin. Shows ~50% crossreactivity with recombinant human Draxin. Species Crossreactivity: rat.

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