DRIP-130 (Vitamin D Receptor Interacting Protein 130, Sur2, CRSP130, ARC130, NAT140, TRAP150b)

DRIPs are a distinct set of ligand-dependent proteins that interact with the vitamin D receptor (VDR). Together, these proteins constitute a new cofactor complex. DRIPs bind to several nuclear receptors to mediate ligand-dependent enhancement of transcription by VDR and the thyroid-hormone receptor in cell-free transcription assays. The DRIPs are almost indistinguishable from components of another cofactor complex called ARC, which is recruited by other types of transcription activators to mediate transactivation on chromatin-assembled templates. The role of nuclear-receptor ligands may, in part, be to recruit such a cofactor complex to the receptor and, in doing so, to enhance transcription of target genes. In humans, interaction with Sur-2 is required for transcription to be activated by the activation domain of a transcription factor of the ETS-family in response to activated mitogen-activated protein (MAP) kinase.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Detects a 130kD band\nImmunoprecipitation: 1:100-1:200. Pre-clearing of the lysate with a non-specific rabbit IgG is helpful to reduce background.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9475

Size

100ul

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.

P Type

Pab

Purity

Purified serum

Isotype

IgG

References

1. Boyer, T.G., et al., Nature 399(6733): 276-279 (1999). 2. Rachez, C., et al., Nature 398(6730): 824-828 (1999). 3. Ko, L., et al., Proc. Natl. Acad. Sci. USA 97(11): 6212-6217 (2000). 4. Chiba, N., et al., J. Biol. Chem. 275(15): 10,719-10,722 (2000). 5. Hittelman, A.B., et al., EMBO J. 18(19): 5380-5388 (1999).

Additional Info

Recognizes mouse DRIP-130 (vitamin-D-receptor interacting protein 130). Used to detect DRIP130 using nuclear extracts from mouse thymus, spleen and brain. Species Crossreactivity: Human.