DSCAM (Down Syndrome Cell Adhesion Molecule)

Human DSCAM (Down syndrome cell adhesion molecule) is a 230 kD axonal guidance molecule that is a member of the Ig superfamily. It is synthesized as a mature, 2005 amino acid (aa) type I transmembrane glycoprotein. There is a large, 1578 aa extracellular domain (ECD) and a 389 aa cytoplasmic region. The ECD shows ten C2-type Ig-like domains and six fibronectin type III repeats. One soluble splice form is known. This contains all the ECD modules and ends after aa 1571 of the precursor. This form has a unique C-terminal ten amino acids. DSCAM is found on axonal and dendritic processes. It also forms homophilic complexes among cells. Overexpression is associated with Down's syndrome. The ECD of human is 98% and 77% aa identical to mouse and zebrafish DSCAM ECD, respectively.\n\nHybridoma: \nProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human DSCAM (rhDSCAM; aa 18-1595: Accession #NP001380).\n\nApplications: \nWestern Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human DSCAM. Using a colorimetric detection system, the detection limit for rhDSCAM is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. \nDirect ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human DSCAM. The detection limit for rhDSCAM is approximately 3 ng/well.\nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D9635-50

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

Additional Info

Selected for its ability to detect human DSCAM in direct ELISAs and Western Blots. In these formats, this antibody shows no crossreactivity with rhDSCAM-L1.