Duodenal Cytochrome b (Dcytb)

Iron is an essential element for a variety physiological process. A variety of proteins are involved in the transport of iron. Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1. These transporters are especially active in small intestine where most dietary iron absorption is conducted. Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III). Therefore, highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 (ECL).\nELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide:\nD9830: Duodenal Cytochrome b, Mouse, Control Peptide (Dcytb)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D9830-25

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. McKie, A.T., et al., Science 291: 1755-1759 (2001). 2. Escriou, V., et al., J. Biol. Chem. 269: 14007 (1994). 3. Royer-pokora, B., et al., Nature 322: 32-38 (1986). 4. Dinauer, M.C., et al., Nature 327: 717-720 (1987).

Additional Info

Recognizes mouse Duodenal Cytochrome b (Dcytb). No significant sequene homology exists with b558 or other reductases.