DUSP3 (Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase)

DUSP3(Dual-specificity phosphatase 3), also called VHR, is a member of the dual specificity protein \nphosphatase subfamily. DUSPs constitute a large heterogeneous subgroup of the type I cysteine-based \nprotein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate \nboth tyrosine and serine/threonine residues. DUSP3 contains the consensus DUSP C-terminal catalytic \ndomain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase \nphosphatase) class of DUSPs. The DUSP3 gene is mapped on 17q21.31. Confocal microscopy \ndemonstrated that phosphorylated VHR accumulated at the immune synapse between the T cell and \nthe antigen-presenting cell in the presence of antigen. Tyrosine phosphorylation of VHR affects \nprotein-protein interaction, subcellular location, or substrate targeting, given that tyr138 is located on \nthe opposite side of the VHR catalytic center.\n\nUniProt Number:\nP51452\n\nGene ID:\nDUSP3\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144462

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Alonso, A., Rahmouni, S., Williams, S., van Stipdonk, M., Jaroszewski, L., Godzik, A., Abraham, R. \nT., Schoenberger, S. P., Mustelin, T. Tyrosine phosphorylation of VHR phosphatase by \nZAP-70. Nature Immun. 4: 44-48, 2003.\n2. Ishibashi, T., Bottaro, D. P., Chan, A., Miki, T., Aaronson, S. A. Expression cloning of a human \ndual-specificity phosphatase. Proc. Nat. Acad. Sci. 89: 12170-12174, 1992.\n3. Patterson, K. I., Brummer, T., O'Brien, P. M., Daly, R. J. Dual-specificity phosphatases: critical \nregulators with diverse cellular targets.Biochem. J. 418: 475-489, 2009.

Additional Info

Recognizes human, mouse and rat DUSP3. No crossreactivity with other proteins.