DVL3 (Dishevelled-3, Dsh Homolog 3, Segment Polarity Protein Dishevelled Homolog DVL-3, KIAA0208)

The human DVL-3 gene encodes a predicted 716 amino acid protein which exhibits 98% amino acid identity with mouse Dsh-3 and 49% with Drosophila Dsh. Dsh-3 was mapped to 3q27. The expression of Dsh-3 mRNA was detected in 30 human cell lines and 2 primary cell \ncultures. Dsh-3 mRNA was detected in normal human breast tissues and tumors. Statistically, there was no difference in Dsh-3 mRNA level between normal breast tissues and tumors. In human colorectal samples, Dsh-3 was expressed equally in matched normal tissues, polyps and tumors. The data indicates that Dsh-3 is widely expressed in human cells and may have a widespread role in signal transduction (1). Results indicate that the human dishevelled genes constitute a multigene family and that Dsh 3 proteins are highly conserved among metazoans. \n \nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:500-1000 \nImmunohistochemistry: 1:100-250 \nImmunocytochemistry: 1:100-250 \nFlow Cytometry: 1:30 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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