Dynactin (Dynactin Subunit 1, 150kD Dynein-associated Polypeptide, DAP-150, DP-150, p135, p150-glued, DCTN1)

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA).\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:128,000\nWestern Blot: 0.5-2ug/ml ~150kD band observed in cell lline A549 and in Human Testis lysates (calculated MW of 142kD according to NP_004073.2).\nImmunohistochemistry: 2-4ug/ml In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D9894-60B

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1.Bupha-Intr T, Haizlip KM, Janssen PM.Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro.Am J Physiol Heart Circ Physiol. 2009 Mar;296(3):H806-14.\nPMID: 19136602 2.Magnani E, Fan J, Gasparini L, Golding M, Williams M, Schiavo G, Goedert M, Amos LA, Spillantini MG.Interaction of tau protein with the dynactin complex.EMBO J. 2007 Oct 31;26(21):4546-54.

Additional Info

Recognizes human Dynactin 1. Species sequence homology: mouse. This antibody is expected to recognise all reported isoforms (NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1).

Alternative Names

p150, Glued homolog, dynactin 1, OTTHUMP00000202491, HMN7B, dynactin 1 (p150, Glued (Drosophila) homolog), 150kD dynein-associated polypeptide, p150, Glued (Drosophila) homolog, p150-glued, DAP-150, DP-150, P135, dynactin 1 (p150, glued homolog, Drosophila), DCTN1